APEDA - CDFD Centre for Basmati DNA analysis
Agricultural and Processed Food Products Export Development Authority (APEDA) - Centre for DNA Fingerprinting & Diagnostics (CDFD) Centre for Basmati DNA analysis was established in CDFD in 2005 to test Basmati rice samples using the DNA protocol developed at CDFD. The initial funds for establishment of the laboratory have been provided by Basmati Export Development Foundation (BEDF) managed by APEDA, Ministry of Commerce, Government of India.
At the APEDA-CDFD centre, the Basmati rice samples, received from Export Inspection Council (EIC), Ministry of commerce, Govt. of India, Basmati rice exporters and Basmati importers from India and other countries, are tested for their purity by using the DNA protocol developed and standardized at CDFD. The Basmati DNA Test Report indicating the level of purity of Basmati rice in a given sample and the extent of adulteration with non-Basmati rice grains, if any, will be indicated
₹ 12,000/- (Rupees twelve thousands only) Plus 18% Service Tax (as per GST) that amounts to ₹ 14,160/- (Rupees fourteen thousand one hundred sixty only)
Management Committee of the APEDA-CDFD Centre for Basmati DNA Analysis
(DBT, Ministry of Science & Technology, Govt. of India)
Agricultural and Processed Food Products Export Development Authority (APEDA)
(Ministry of Commerce & Industry, Govt. of India)
Export Inspection Council of India (EIC)
(Ministry of Commerce & Industry, Govt. of India)
All India Rice Exporters Association
Prof. E.A. Siddiq
Acharya N.G. Ranga Agricultural University and CDFD
Tilda Rice Land Pvt. Limited
Consultant Scientist & Member Convener
Laboratory of Neurospora Genetics
Centre for DNA Fingerprinting and Diagnostics (CDFD)
Sampling procedure and DNA extraction
From the samples received at the Centre, at least 50 g of grains from each sample is powdered and the remaining sample is stored for three months. During this period, if required, the sample is re-used for DNA analysis. From the powdered sample, eight sub-samples of 1 g each is drawn (Fig. 1), from which 100 mg of grain powder is collected for DNA extraction. DNA is isolated using Qiagen DNeasy plant mini kit and quantity and quality of the DNA (at 260/230 and 260/280 absorbance) are verified using UV-Vis spectrophotometer.
The PCR is performed using the "Basmati VerifilerTM Kit" (Labindia) (Licensed by CDFD to Ms. Labindia under license transfer agreement) and the protocol is followed as per the details provided therein. (PDF Basmati Verifiler Kit)
Capillary electrophoresis (CE)
The fluorescently labeled PCR products are mixed with 0.3 µl of GeneScan-500 ROX (6-Carboxy-X-Rhodamine) size standard (Applied Biosystems, USA) and 10.2 µl of Hi-Di Formamide (Applied Biosystems, USA), electrophoresed by capillary electrophoresis on an ABI PRISM 3100 genetic analyzer (Applied Biosystems, USA) according to the manufacturer's instructions. Subsequently, fluorescent DNA fragments are resolved using the GeneScan version 3.7, and allele size and peak-area of the true peaks are determined by Genemapper version 3.7 (Applied Biosystems, USA).
Note: - In case of adulterated samples, each experiment is repeated thrice, starting from the DNA extraction, to confirm the extent of adulteration of Basmati rice with non-basmati rice grains.
Quantification of adulteration
The quantity of an amplified PCR product is represented by peak area (measured in relative fluorescent units, rfu). The adulteration is expressed as a percent fraction to that of pure basmati:
A is the rfu of the adulterant and
B is the rfu of the main variety.
Issue of Basmati DNA Test Report
Once the DNA analysis of a given rice sample is completed, the duly signed authenticity certificate, which contains the details of percentage of adulterated rice present in Basmati rice sample, is provided to the sender. The scanned copy of the certificate may also be sent by e-mail on request. The certificate is formatted as per the guidelines stipulated by the management committee of the APEDA-CDFD centre.
Fig. 1 Picture showing the protocol followed at the APEDA-CDFD centre for Basmati DNA analysis
IS 16538 : 2019, Microsatellite Based PCR Protocol for Identification of Basmati Rice Varieties, Bureau of Indian Standards, 2019
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