This invention is described
in the following filings:
Novel FISSR-PCR primers and methods of identifying genotyping
diverse genomes of plant and animal systems including rice varieties,
a kit thereof.
The present invention relates to set of 37 inter-simple sequence
repeats (ISSR)-PCR primers for genotyping eukaryotes and a method of
genotyping diverse genomes of plant and animal systems using FISSR-PCR
primers and SSR markers; more particularly, a FISSR and SSR method of
distinguishing Basmati rice varieties from Non-Basmati (NB) rice varieties,
and Traditional Basmati (TB) rice varieties from Evolved Basmati (NB)
rice varieties, and also, a method for determining adulteration of Basmati
rice with other rice varieties and a kit thereof.
2. USPTO – Continuation-in-part
Single tube multiplex assay for detection of adulterants in basmati
J. Nagaraju et al.
The present invention relates to a single tube multiplex assay
for distinguishing basmati from non-basmati rice varieties and thereby
the adulteration, said assay comprising steps of running multiplex PCR
with sample using eight loci, and distinguishing the basmati from non-basmati
rice varieties and thereby the adulteration on the basis of varietal
specific multiplex allele profile; and also, a method of quantifying
adulteration in basmati rice varieties, said method comprising steps
of constructing a standard curve on the basis of ratio of quantity of
amplified products of the alleles of adulterant and the basmati rice
against the progressive proportion of adulteration, and quantifying
the adulteration in basmati rice variety on the basis of peak area of
the alleles corresponding to basmati and that of the adulterant.
L.D., Kathirvel, M., Rao, G.V., and Nagaraju, J. 2001. DNA profiling
of disputed chilli samples