Centre for DNA Fingerprinting and Diagnostics

Bldg. 7, Gruhakalpa, 5-4-399 / B, Nampally, Hyderabad - 500 001, India

Tel: 040-24749381




RATE LIST

Sl.No.
Equipment (with make/ model)
Services offered
Sample types
Sample concentration / volume
 Cost (in INR)
Academia Corporate
GENOMICS:
1.

DNA Sequencers
(ABI 3130 xl GA)

 Sequencing DNA template and primer
PCR product: 50 ng/µl
Plasmid: 150-200 ng/µl
Primers: 50 pmol/µl
 Rs. 300 per sample Rs. 500 per sample
Genotyping Processed samples NA Rs. 90 per sample Rs. 180 per sample
2.

Micro array scanner Gene Pix 4000B
(AXON)

 Expression analysis, pathogen detection Processed slides NA Rs. 720 per hr. Rs. 1500 per hr.
3.

Real Time PCR
(ABI 7000)

 Plate reading Processed plate NA Rs. 650 per plate Rs. 1500 per plate
Setting up reactions 1-10 samples DNA/cDNA template and primers Standardized by PCR Rs. 270 per sample Rs. 600 per sample
Half plate Rs. 10800 Rs. 25000
Full plate Rs. 18000 Rs. 37500
4.

DHPLC
(Transgenomics)

 Mutation analysis, fragment size DNA/cDNA PCR amplified 20 ng/µl~50µl Rs. 126 per run Rs. 270 per run
5.

SOLID
(ABI)

 Next Generation sequencer Installation and validation  in progress
CELLOMICS:
6.

Confocal Microscope
(LSM 510 META) Zeiss

 Fluorescence, Live cell imaging, time lapse and FRET, FRAP

Fixed slides OR
Chamber slides

 NA Rs. 1800 per hr. Rs. 3500 per hr.
7. Live Cell Imaging Live cell imaging, time lapse Installation and validation in progress
8.

FACS  Vantage SE
(Becton Dickinson)

 Fluorescence, cell cycle Flourchrome labeled cells NA
1-5 samples Rs. 750 per sample Rs. 1500 per sample
6-10 samples Rs. 550 per sample Rs. 1150 per sample
11-16 samples Rs. 360 per sample Rs. 750 per sample
Above 16 Rs. 180 per sample Rs. 400 per sample
9.

FACS ARIA
(Becton Dickinson)

 Cell sorting Labeled cells NA To be decided
PROTEOMICS:
10.

X-ray crystallography
(Bruker AXS and MAR 345 Dtb)

Preliminary scanning
(1 hour)

 Single crystal NA Rs. 450 per crystal Rs. 1500 per crystal
Full spectrum NA Rs. 3600 per 8 hr. Rs. 10000 per 8 hr.
Data analysis Diffracted crystal data NA 15000 per crystal 40000 per crystal
11.

CD Spectropolarimeter
(Jasco J-810)

 Structure analysis Protein sample 1 mg/ml~200-300 µl Rs. 750 per hr. Rs. 1500 per hr.
12.

MALDI-TOF/ TOF
MS
Daltonics
ULTRAFLEX III
(Bruker)

 Molecular weight analysis Proteins in solution 1-2 µg/µl~5µl Rs. 900 per sample Rs. 2000 per sample
Peptide Mass Fingerprint (PMF) and sequencing of at least 3 peptides Pure proteins in solution/ 1D or 2D gel plug 1-2 µg/µl~5µl
or
0.1 – 2 µg/gel plug		 Rs. 1800 per sample Rs. 3750 per sample
Data analysis Spectra NA Rs. 900 per sample Rs. 2000 per sample
LC-MALDI Protein/ peptide mixture 1-5 µg/µl~10µl Rs. 9000 per sample Rs. 20000 per sample
13.

BIACORE 3000
(GE Healthcare)

 Protein-protein, protein-drug, protein-nucleic acids binding constant analysis Proteins, peptides, small molecules etc.

Ligand: 10-50 µg/µl
50-100 µl for immobilization

Analyte: 10-50 µg/ µl~1 ml

 Rs. 750 per 4 runs Rs. 2000 per 4 runs

*Chip costs have to borne separately
14.

Two Dimensional Electrophoresis
(BIORAD)

 Protein profiling Protein sample 100-500ug/gel Rs 1800 per sample Rs 3750 per sample
15.

High Pressure Liquid Chromatography
(Agilent 1100)

 Amino acid analysis Protein, spent media, body fluids ~300 µl Rs. 720 per sample Rs. 1500 per sample
Size exclusion Protein samples 1 mg/ml ~100 µl Rs 200 per run Rs. 400 per run
Reverse phase separation and purification Proteins, peptides, small molecules etc. 0.2 µg/µl Rs 125 per run Rs 300 per run
16.

Gas Chromatography- Mass Spectroscopy
(Shimadzu 2010)

 Only Run Processed sample 100nmols Rs 198 per sample Rs 412 per sample
Amino acid analysis Protein sample Rs 720 per sample Rs 1500 per sample
Carbohydrate analysis Extracted Sample Rs 900 per sample Rs 2000 per sample
Fatty acid analysis Extracted sample Rs 2700 per sample Rs 5600 per sample
Organic Acid analysis Extracted sample Rs 900 per sample Rs 2000 per sample

* - Any bulk samples or special requests may be communicated to Director, CDFD

   - Director, CDFD reserves the rights to process or reject requests for processing the samples